사람 치주인대섬유모세포에서 MMP-1, TIMP-1,2, Type 1 collagen, fibronectin 및 UNCL 발현에 미치는 H2O2와 chlorhexidine의 효과
Effects of H2O2 and chlorhexidine on MMP-1, TIMP-1,2, Type 1 collagen, fibronectin and UNCL expressions in human periodontal Ligament fibroblasts
최성미, 장현선, 김병옥,
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최성미 ( Choi Seong-Mi ) - 조선대학교 치의학전문대학원 치주과학교실
장현선 ( Jang Hyun-Seon ) - 조선대학교 치의학전문대학원 치주과학교실
김병옥 ( Kim Byung-Ock ) - 조선대학교 치의학전문대학원 치주과학교실
KMID : 0363020080380040645
Abstract
Purpose: To evaulate the effects of chlorhexidine and on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase(TIMP-1, TIMP-2), Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts (hPDLF).
Materials and Methods: , and CHX and , and and mixture of CHX and were applied to hPDLF for 1 min and 30 min. The mRNA expressions of MMP-1, TIMP-1 and 2, Type 1 collagen, fibronectin and UNCL in hPDLF were analysed by RT-PCR.
Results: The result were as follows: 1. The expression of UNCL mRNA was higher than that of other mRNAs. 2. CHX increased mRNA expressions of hPDLF as application time increased. 3. lower than increased expression of UNCL mRNA, and did not decrease mRNA expression of hPDLF. 4. hPDLF treatment with CHX (with or without ) resulted in no gene expression. 5. hPDLF treatment with CHX (with or without ) for 30 minutes resulted in no gene expression.
Conclusion: Because low concentration of CHX and increased UNCL mRNA expression of hPDLF, low concentraction of CHX and may have an antioxidative effect.
키워드
chlorhexidine;H2O2;human periodontal Ligament fibroblasts
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